FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34490702
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA41700.016.95974549
GCCGTAT45200.015.95178847
TATCGCG10600.015.62212220
TCGCTCG50350.013.361588514
CGCCGTA56900.012.9142446
CGAAGAT110600.011.9046361
TTACACG39500.011.52868132
CCGTATC62900.011.4629748
GCGTCGT67350.011.269032511
TAAGCGA19100.011.19850146
GATCGGA78850.010.9568221
CGCCAAT65350.010.4704641
ACTCGTG66600.010.20460122
GTAGATC80700.010.00334231
CCCGATA66850.09.95995669
CGCAGAC111800.09.7985941
CGCGGAT32500.09.7825211
CCGGGAT85050.09.7111331
ACCCGAT67500.09.71071868
TAAGTCG27200.09.38571720
Produced by FastQC (version 0.11.5)