FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27410982
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37800.022.27726245
TATGCCG39100.021.09764948
CGTTTCG18150.020.14356233
CGTATGC42100.020.08498646
ACACGTC52600.017.37713813
GATCGGA48200.015.7698351
CTCGTAT55700.015.55009844
ACGTCTG59500.015.303390515
CACGTCT65350.014.19761214
AGTCACG42050.014.10807928
ATCGGAA57900.013.12719252
TCTCGTA66400.012.93427943
TTACACG29400.012.90485532
GTCACGT45000.012.64685129
CGCGAAA11400.012.40480643
CGTACGT8200.012.19813734
ATCTCGT69400.012.12675242
CACACGT84450.011.06847412
CGTATCG18450.011.0479733
AATCTCG42700.010.90485941
Produced by FastQC (version 0.11.5)