FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27410982
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG7450.014.3545222
CGTATCG18550.012.0970693
GCGTCGT24850.012.07789212
TCGCTCG35800.012.04357714
CGCGGGT26300.011.6821931
CGTATCA25400.011.54427950
TTACACG31700.011.53654732
CTCGGGT61300.011.3757741
TCGGGTC51500.011.060582
CGAAGAT90800.010.6834261
CTCGGAT66450.010.4941311
CGCGAAA9600.010.42160343
GCCGTAT28600.010.37277648
CGCGATT7503.6379788E-1210.1263171
GCGCTAA15450.010.04831942
GTATCGA23550.09.968624
CGCGCTA15050.09.62744646
CTCGGTT34750.09.4375421
CCCGTAT32200.09.4344571
AGTCGCG12150.09.3696486
Produced by FastQC (version 0.11.5)