FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29892060
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA17500.018.92703615
CGGCATA23950.018.00755312
GCATACG25650.016.94858414
TCGCTCG35750.013.22192614
TAAGCGA19150.012.24646846
CGAAGAT94400.010.7435311
CGCTCGT46600.010.58764915
CGCAGAC71500.09.890531
CGGGGAT51250.09.759911
CCGGGAT50200.09.6891811
TTACACG31350.09.57898332
GATCGGA45850.09.4798821
CGCGTTC17850.09.27797514
ACTCGTG55700.09.22579922
CGGAGAT78800.09.1493821
CGTTCGC16650.09.11775416
CGGGTAT16650.09.1161211
TCGGGTC47400.09.0970782
CCGGCAT50450.08.95739651
CTCGGAT67100.08.8425521
Produced by FastQC (version 0.11.5)