FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39922531
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG71100.016.68773543
TATGCCG71250.016.5549846
CGTATGC77100.015.34425944
CTCGTAT82850.014.82792142
ACACGTC94050.013.061213513
GATCGGA90300.012.5420421
ACGTCTG107650.011.346115
TCTCGTA102500.011.33638141
ATCTCGT108500.010.96735140
ATCGGAA105450.010.8526642
CACGTCT118050.010.67263514
TACCGTA29350.010.3740029
ATGCCGT118800.010.37067747
CGCGAAA16150.010.1857443
CGTTCGC31800.09.79485716
ACCGTAT29000.09.65444210
CGACCGT19350.09.422711
CGCGTTC34400.09.35975614
CTCGTTT63900.09.3282791
CCGGGAT74650.09.3001271
Produced by FastQC (version 0.11.5)