FastQCFastQC Report
Thu 2 May 2024
EGAF00004858831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28978046
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG427280.1474495554324125TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC420480.14510295138602514TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)