FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28978046
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG101550.032.725581
TCGTATG119900.030.9889143
CGTATGC125100.029.7559644
TATGCCG126450.029.46556346
ACACGTC137250.027.19779413
TATCTCG133700.027.09361339
CTCGTAT141200.026.72959942
GATCGGA134300.025.8758371
ACCGATG144150.025.15342532
CGATGTA145050.024.73572734
CACGTCT154750.024.45606214
ACGTCTG157050.023.94413415
CACCGAT149650.023.92928331
TCTCGTA157100.023.7827141
ATCTCGT159350.023.64174840
ATGCCGT159550.023.41755147
CCGATGT157700.022.81716533
CACACGT167000.022.3320212
ATGTATC171450.021.04763236
TCACCGA172750.021.02903730
Produced by FastQC (version 0.11.5)