FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16475259
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54650.038.40263748
TCGTATG54750.038.39482545
CGTATGC56450.037.1781146
TATCTCG60100.034.06849341
GATCGGA67750.032.5140231
ACACGTC68100.031.77431913
ACGTCTG72100.030.05804415
CTCGTAT69650.030.03415944
TCTCGTA72400.028.37488443
ATCGGAA77800.028.3371872
ATGCCGT74100.028.32259449
CACGTCT77800.028.16369214
ATCTCGT73550.027.69923442
GCCGTCT80050.026.26004651
ATATATC78900.025.77777338
CACACGT84900.025.64754512
CCGTCTT82350.025.31941852
GATATAT82150.025.21490137
TCGGAAG88850.025.1201843
GCACACG92900.023.84304811
Produced by FastQC (version 0.11.5)