FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16475259
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA27350.022.16124249
GATCGGA51100.020.2835271
GCCGTAT31250.019.94610247
GCGTCGT35700.019.61600111
ATCGGAA57550.017.9506592
GTCGCCG35100.017.5661144
AGCGTCG48150.016.99168210
CGCCGTA37150.016.8744346
AGAGCGT49300.016.2400078
TTACACG20150.015.83320432
GGTCGCC46650.015.21056343
CCGTATC41200.015.12888548
TCGGAAG68050.014.8272213
AAGAGCG62150.014.7783487
GTAGATC50350.014.54358831
CGTCGTG51200.014.4223812
GATCTCG63600.013.750734
GGCGCCG26800.013.49535144
TGGTCGC36000.013.49213242
ATCTCGG70300.013.18038235
Produced by FastQC (version 0.11.5)