FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31956621
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG56800.027.05214943
TATGCCG58050.026.88234146
CGTATGC63350.024.63330544
TATCTCG66900.022.6610539
AGTCACG71000.021.8844328
ACACGTC77050.020.96395113
GATCGGA70700.020.774791
CTCGTAT78900.020.08219342
ACGTCTG84850.019.0369515
CGCCAAT88950.017.92829533
CACGTCT93300.017.53288714
ATCGGAA83700.017.4423642
TCTCGTA91950.016.67375641
ATGCCGT96500.016.66767347
ATCTCGT92150.016.60024840
CACACGT107800.015.17444812
TCGGAAG106500.013.8835683
ACGCCAA112300.013.83487132
GCACACG125750.013.8248211
GCCGTCT115750.013.45218449
Produced by FastQC (version 0.11.5)