FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31956621
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA34100.017.30188249
GCCGTAT41550.014.8639347
GCGTCGT48650.014.75062311
GATCGGA64700.013.7672131
TTACACG31900.013.73794532
CGCCGTA48450.012.81317846
TCGCTCG39800.011.78818314
AGCGTCG70350.011.57384710
CGAAGAT105300.011.2131681
ATCGGAA79050.011.2109242
AGAGCGT70650.011.0358478
TATCGCG7350.010.79596520
CGCGAAT14050.010.5662951
GTAGATC70550.010.56490231
CGCGTAT4956.497066E-810.4620471
CCGTATC60500.010.20820348
GATCTCG93900.09.48129534
GTATCGA29050.09.3777344
AAGAGCG92300.09.3439997
CGCCAAT53650.09.3310151
Produced by FastQC (version 0.11.5)