FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39605332
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA14900.012.45028943
CTCGGGT87200.010.0912911
CGTTCGC23650.010.06241716
CTCGGTT47350.09.9954721
TCGGGTC68500.09.7581462
CCCGTAT45900.09.4710581
CGCGGGT29650.09.45920851
CGCGTTC26150.09.23425714
CTCGGAT91350.08.9420461
CGTATCG33800.08.9044343
CTCGTTT58850.08.8762461
CCGCGTT29400.08.80864213
TACCGTA28950.08.7037929
CGCGTAA5251.3607787E-58.66579910
CTCGGAG156500.08.4901311
CGACCGT18250.08.4523861
CTCGACT47700.08.4521851
CCGGGAT71700.08.3611591
CCGGCAT60800.08.0149491
CGAAGAT141800.07.9857521
Produced by FastQC (version 0.11.5)