FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39605332
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA15850.014.57410243
CGTATCG35000.012.19780353
CCCGTAT45850.011.3719551
GTATCGA43000.010.9049574
CTCGGGT88450.010.840291
TCGGGTC70400.010.5875422
TTACACG45150.010.46381332
CTCGTTT60550.09.8825761
CGAAGAT138200.09.495361
ATCGCGC35350.09.308228567
CCGCGAA26550.09.22778342
CTCGGAT89550.09.2222011
CGTTCGC26450.08.99801316
CTCGGTT50250.08.9834231
CTCGACT50300.08.9744941
TATATCG26150.08.96666235
CCCGGTT46050.08.73889351
TACCGTA29000.08.6885519
CCGGGAT71850.08.6205441
CTCGTAT46300.08.6161261
Produced by FastQC (version 0.11.5)