FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22014319
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA90300.035.72136350
AGATCGG95650.034.980811
GTCGCCG93500.034.6109245
GCCGTAT93950.034.3321748
GCGTCGT96250.033.9388112
CGCCGTA97650.032.9989847
TGGTCGC96550.032.77658543
TCGCCGT98650.031.82692346
CCGTATC103550.031.38290649
AGCGTCG108100.030.41088511
TCTCGGT115900.028.83997537
CGGTGGT112200.028.50279240
CGTCGTG116000.028.3995913
GGTCGCC114650.028.2027944
AGAGCGT118300.027.9910629
GTAGATC119250.027.61819332
GATCGGA124050.027.388732
TCGGTGG123850.026.98854339
ACGTACG13300.026.95450826
CGTGTAG123100.026.35585616
Produced by FastQC (version 0.11.5)