FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23784134
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24100.029.48615843
CGTATGC27200.025.36466244
TATGCCG28250.024.66612246
GATCGGA34300.020.458331
ACACGTC36150.020.13519313
TATCGCG20150.018.83192622
CTCGTAT38850.017.66973342
CACGATC41150.015.50863536
ATCGGAA45650.015.1915712
CACGTCT47950.015.10828314
ACGTCTG49250.014.779531515
ACGATCT46050.014.15791337
ATGCCGT55000.013.54729747
TCACGAT53850.013.52043735
GCGGTAT37450.013.1993481
CGTATCG21500.012.6757863
TCTCGTA51600.012.56828841
ATATCGC33600.011.8068221
ATCTCGT55700.011.76701840
CGCGTAT3551.4363468E-611.6847961
Produced by FastQC (version 0.11.5)