FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23784134
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG20700.015.33899622
CGTATCG22050.013.1468813
GTATCGA24350.012.8967644
CGCGTAA10300.011.3918351
CCCGTAT28050.011.0729071
TTACGCG9550.010.83171154
ATATCGC32350.010.24169521
ATCGAAG43550.010.2222016
CGTTCGC15600.010.17028116
CGCTAAT14000.010.10045634
CTAACGG10700.09.69467669
CGCGGAT19700.09.6349291
GCGGTAT35900.09.612971
TCGGGTC48900.09.4568822
ACTAACG11750.09.41590768
GCGGGTT30800.09.4119721
CCGTATC39050.09.3677842
CGTTCTA15550.09.31841544
GTCGCTA15900.09.11703427
CTCGGGT56100.09.0428741
Produced by FastQC (version 0.11.5)