FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29912794
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAT76650.017.94909128
GAACGCT76900.017.7830735
ATCGAAG94950.017.5307586
CCGACAT84000.017.3945431
GTCGCTA84450.016.45217127
AAGCGAC82450.016.37170220
CGCTATG85900.016.01627229
CGTATCA27300.015.67860950
CGACATC95400.015.489612
GCGGTAT76850.015.3787351
AAAAGCG89800.015.13154418
TTACGCG47800.014.85325454
CGAAGGA97850.014.8240438
TCGAAGG112900.014.6532737
TACGCGA50800.014.24357855
ATCGGTA33050.013.97870818
TTGGCCG101050.013.68255241
GACATCG110600.013.6372713
ACGCTTG103800.013.40500637
ACATCGA106750.013.3653714
Produced by FastQC (version 0.11.5)