FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31951537
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG69100.042.8330243
CGTATGC73900.040.09757644
TATGCCG75350.039.23439846
ACACGTC88300.035.01132213
GATCGGA89450.033.5451741
CTCGTAT86500.033.41924742
CACGTCT101750.030.2476514
TCTCGTA100650.027.65849341
ATGCCGT108650.027.59049447
ACGTCTG121450.025.36968815
CACACGT122400.025.22754312
TCGGAAG121300.025.0820833
ATCTCGT115150.024.17566140
GCCGTCT130550.022.96210349
ATCGGAA132350.022.6525762
GATCTCG127200.022.26510639
GCACACG144000.022.15746711
TGCCGTC142900.021.09835648
GTCACAC150600.020.1160329
CGGAAGA166000.019.2627354
Produced by FastQC (version 0.11.5)