FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31951537
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA28750.018.36196550
CGTATCG36350.016.7074663
GCGTCGT34750.015.48176112
CGCGTAA27700.015.44697951
CCCGTAT39150.014.3667151
GTATCGA36600.013.9534934
GCGGTAT51850.013.50981
TTACGCG19700.012.60039654
GCCGTAT42450.012.27328348
AATCGGG55250.011.6796025
ATCGAAG71250.011.6721696
CCGTATC62000.011.6320692
GATCGGA48050.011.2747932
CAATCGG46300.011.2537774
TCGATTG37500.011.23897620
CGGTATA23350.011.2312752
CGGAAAT59950.011.1664271
TACTCGA42000.010.75739917
CGAGCGA57300.010.65239853
TACGCGA24450.010.5743655