FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33671121
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG35950.017.0304743
GTATCGA35650.016.3602924
CCCGTAT37200.015.8872231
CGCGTAA14150.015.2917851
CCGTATC50750.013.0163692
GTCGCTA17850.012.459990527
TTACACG33300.012.29586932
ATCGAAG70000.012.0147456
CCGGTAT43700.011.2331611
TATCGAA48000.010.9425475
CGGAGAT90900.010.7295851
GACGCTA35450.010.7260014
CGCGCGT24450.010.6990691
CGGTATT39850.010.5929032
TCGGGTC63500.010.4028452
ATCGCGC40750.010.36013467
GCCGTAT26600.010.3199381
CGGAAAT58550.010.2594921
GCGCTAA19850.010.22395142
GTACGAT26550.010.196156566
Produced by FastQC (version 0.11.5)