FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24071820
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG62050.045.8863843
CGTATGC67700.042.17688844
CTCGTAT70850.039.66958242
TATCTCG70900.038.72247739
TATGCCG77250.038.3971446
ACCGATG93900.033.02740532
ACACGTC95700.032.70063413
TCTCGTA86400.032.19996341
GATCGGA93650.031.8351141
CACCGAT100350.031.24045831
CGATGTA95700.030.74381634
ATGCCGT99400.029.90896447
ATCTCGT96100.029.03465540
CCGATGT102850.028.73798233
CACGTCT112050.028.1104114
ACGTCTG114400.027.46245615
GTCACCG117200.026.69081329
TCACCGA117800.026.55486930
CACACGT122650.025.54290612
GCCGTCT117850.025.51606249
Produced by FastQC (version 0.11.5)