FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30719031
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT29150.034.95239335
CCGTACG35450.030.32203734
TACGTAA36100.028.58816737
ACCGTAC39600.027.3941433
GTACGTA38750.026.71863236
TCGTATG60250.024.90490346
TATGCCG61700.024.42727349
CGTAATC41100.024.38711739
CGTATGC64250.023.55975547
ACGTAAT45000.022.78637138
GATCGGA70450.021.6707862
ACACGTC79600.020.50276214
AGATCGG76650.019.8373811
CTCGTAT80550.018.50500745
ATCGGAA82850.018.3076633
TCTCGTA85000.017.80762144
ACGTCTG95750.017.4577116
CACCGTA65400.017.0914532
CACGTCT97700.016.9405415
AATCTCG64500.016.86855942
Produced by FastQC (version 0.11.5)