FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30719031
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA43150.031.9152550
GCCGTAT45600.030.63820548
GCGTCGT50950.030.49601612
CGCCGTA49550.028.59956447
GATCGGA60500.027.6152422
AGCGTCG64700.024.42729811
CCGTATC60100.023.35668249
ATCGGAA72550.023.1202683
AGAGCGT72450.022.2284629
TGGTCGC64750.021.62860943
GTCGCCG67900.021.45896545
CGTGTAG78250.020.57448616
TCGCCGT67400.020.53056746
AGATCGG79450.020.5243511
GTCGTGT78000.020.51216114
TCGTGTA77600.020.27376715
CGTCGTG82100.019.2493813
GTAGATC82650.019.12028732
GGTCGCC79550.019.07030944
GTATCAT76650.018.66136751
Produced by FastQC (version 0.11.5)