FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25694313
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA22900.014.91390350
GCGTCGT26300.014.297852512
GCCGTAT26300.013.77246448
CGACCTA17600.012.5533144
CGCCGTA28900.012.53285747
GATCGGA34200.011.9102932
TATCGCG6750.011.24353622
CCCGTAT31500.010.9583451
TTACACG30100.010.77428532
CTCGGAT62550.010.540511
CGAAGAT84100.010.30227851
GTTCGAT27950.010.2506611
CTCGGGT56150.010.1435451
CGTATCG20450.010.1282163
TAAGTCG25450.09.894670520
CTCGGTT30700.09.7821971
CATACGA13450.09.74566615
TCGCTCG33000.09.61652514
CGCGGAT24100.09.596511
CGCGAAT13700.09.5745541
Produced by FastQC (version 0.11.5)