FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26076064
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT16000.011.3773961
CCGGGAT46050.010.5668281
CGGGTAT14300.010.2818831
CTCGGGT51500.09.7204921
CGCGTAT4005.9195118E-59.6270271
CGCGCAT7655.2750693E-119.6098651
CTCGTTT40550.09.5827821
CCGCGTT19550.09.4889613
CGCGTTC18300.09.37204814
CGACCGT10250.09.2214541
TTACACG28150.09.20204832
CGCGTTT13850.09.0993881
CGTTCGC17900.08.99485916
CGCTAAT9751.8189894E-128.9762491
CTCGGTT33650.08.9469061
CGAAGAT85350.08.9415431
CTCGGAT58750.08.9380521
GTCGTTA17350.08.87529520
CGCGCTA11850.08.86026346
TCGGGTC42350.08.8431922
Produced by FastQC (version 0.11.5)