FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26076064
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGT57650.010.4424741
TCGCGCG5804.7384674E-910.2594386
CGAAGAT88350.010.0623981
CCGGGAT43450.09.9886361
TTACACG26900.09.887897532
TCGGGTC48150.09.8862382
CGCGAAA9350.09.73201543
CGCGGGT17700.09.6894051
CGTATCG18350.09.5373623
CTCGTAT27300.09.4873161
TCGCTCG28450.09.47173214
CGACCGT11100.09.4595961
CGCGGTT18200.09.4232131
CCCGTAT30850.09.4166681
CTCGAAC55900.09.3919071
CTCAGAT159200.09.2997571
GTATCGA24900.09.2777044
CGGAAAT49800.08.9258311
CGATAAC23250.08.8818481
CGCGCAT8353.0377123E-108.8025221
Produced by FastQC (version 0.11.5)