FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36501965
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC9150.013.7839451
TCGCTCG47000.013.54952114
CGCGTAT5500.013.3766931
TATATCG20400.011.67028535
CGCGCAT13050.011.5438351
CGGAGAT111300.011.3318141
CGAAGAT123850.010.9190141
CGCTCGT57200.010.88865915
CTCGGTT46300.010.5178251
ATATCGC24000.010.357038536
CTCGGAT89350.010.1946171
CGCGGGT27850.010.1894781
CGCAGAC92750.010.0853151
CGGAAAT73400.010.0234071
CGCGACT12350.09.9287191
CGATCCA54850.09.8239514
CCGGGAT66700.09.7696561
CCGGCAT67050.09.5619061
ACTCGTG68750.09.467978522
GTATCGA39650.09.4424074
Produced by FastQC (version 0.11.5)