FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25836509
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG67750.036.06074543
TATGCCG70550.034.44342846
CGTATGC73550.033.45220644
ACACGTC80250.032.36448313
GATCGGA75950.032.2205121
CTCGTAT80350.029.93819242
ACGTCTG91600.028.42777315
ATCGGAA86950.028.2602142
CACGTCT98000.026.88509814
TCTCGTA91850.025.40857741
ATCTCGT97500.024.14645640
ATGCCGT102100.023.90073647
TCGGAAG107150.023.2822513
CACACGT104800.022.75971812
GCCGTCT109150.022.29565849
CGTCTGA117800.022.21914316
CCGTCTT112900.021.7669850
AATCTCG47850.021.20862239
GCACACG117100.020.88415311
TGCCGTC122250.020.493648
Produced by FastQC (version 0.11.5)