FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30901407
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39150.033.81728443
CGTATGC42300.031.87680444
TATGCCG46800.028.86662546
CTCGTAT52650.024.83664542
GATCGGA64350.021.3220631
ACACGTC66100.021.26561513
TCTCGTA70550.017.72679341
ATGCCGT79250.017.34585647
CACGTCT82750.017.31424914
ACGTCTG89400.016.09405915
TTACGCG19000.015.86159354
ATCTCGT82000.015.40056540
CACACGT96350.014.87030912
TCGGAAG95850.014.5593763
ATCGGAA99400.013.7688612
GCCGTCT103650.013.75354949
GCGGTAT58500.013.7250911
TACGCGA22500.013.54473255
GCACACG115500.013.22810411
GATCTCG97800.013.12080739
Produced by FastQC (version 0.11.5)