FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31571991
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA13050.012.60532743
CGTATCG24100.010.74712853
CGCGAGT12700.010.4726431
CCGGGAT55200.010.0817161
GTATCGA32900.010.0001614
CGAAGAT102500.09.8343351
CCCGTAT35250.09.8299751
CGACCGT14650.09.5564991
TCGGGTC56050.09.55415252
CTCGGGT75200.09.5414071
TCGCTCG45650.09.047070514
CGCGGGT25150.09.0458991
TTACACG32300.08.99379532
CCGGCAT54200.08.9761881
GCGTACG14850.08.956202523
CTCGGAT77050.08.9489071
TAAGTCG29050.08.91563720
CGTTCGC23300.08.86263516
CGCGTTC23750.08.84207914
AACGCGC6502.9788862E-78.6154415
Produced by FastQC (version 0.11.5)