FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21815539
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG27050.026.78221343
CGTATGC31200.023.33040844
TATGCCG31150.022.92449646
AGTCACG36000.020.98539528
GATCGGA33950.020.2384971
TATCTCG37200.019.2889239
ACACGTC39400.018.39187613
TATCGCG12950.018.3804822
CTCGTAT43200.017.96773142
CGCCAAT48300.015.85566233
TTACGCG13250.015.100718554
CGCGTAA17350.014.9254261
CACGTCT52550.014.1835214
GTCGCTA17600.014.11222227
TCTCGTA52550.014.04867941
ATGCCGT49850.013.84051847
TACGCGA15150.013.43463655
GTCACGC56400.013.39493329
CGTATCG17150.013.0774293
ACGTCTG58550.012.84414415
Produced by FastQC (version 0.11.5)