FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21815539
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG11850.017.46376622
CGTATCG18650.015.1649183
GCGGTAT31700.014.7262721
CGTATCA17500.013.79637650
GCGTCGT20200.013.14759712
CGCGTAA17500.012.2510111
GTCGCTA18750.011.22105227
CCCGTCG46400.011.1787061
CGAGCGA42350.011.07626956
CGCCGTA23350.010.48760947
ATTCCGT43850.010.38270253
CCCGTAT22750.010.3358411
CGCCGAT29250.010.1669551
CCGGTAT27900.010.1631411
ACGGACA44900.010.13987518
ATCGAAG44250.09.9770866
GCGGTGT42800.09.9375561
CGATCCA28750.09.8374164
GATCGGA25600.09.8353072
CCGTCGG51400.09.7970372
Produced by FastQC (version 0.11.5)