FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32767218
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG16850.015.55135954
CCCGTAT35500.013.8027481
CGCGTAA21000.013.6384291
CGTATCG34200.013.5212273
GTCGCTA23150.013.26839127
GTATCGA34700.012.8291424
TACGCGA21100.012.74493455
ATCGAAG71100.012.1343856
CGATCCA45350.012.0231214
GCGGTAT51050.011.5586191
ACGCGAC26850.010.14394756
CCGTATC54700.09.9679352
TTACACG39150.09.95632432
CCGACAT56550.09.9462771
GTTACGC28600.09.64605253
AATCGGG45950.09.3879745
CCGGGAT63750.09.3642171
TCGCTCG61600.09.18384814
CGAAGAT99250.09.0395821
CGATCGG33750.08.9980074
Produced by FastQC (version 0.11.5)