FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35571689
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37900.013.653346
TCGCTCG39600.012.98040614
CGTATCG28700.012.8639283
TATGCCG42200.012.26224249
CGTATGC44000.012.07406847
GTATCGA33600.011.3979974
CGAAGAT105600.011.1630521
CGCTCGT48200.011.09383915
CGCGTAT5653.237801E-1011.0132761
CGCGCAT10100.010.9526971
CGCAGAC79550.010.2991321
CGACCGT12150.010.24281
GTTCCGT51550.09.83726336
CTCGTAT53850.09.67334845
CGCAAAT46150.09.5131531
CCGGGAT53300.09.4693051
CTCGGAT73150.09.3571431
TCGGGTC53750.09.2461332
CTCGGTT43500.09.218521
CGCGGGT22600.09.177731
Produced by FastQC (version 0.11.5)