FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35571689
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG40250.013.54270914
GTCGCCG39350.012.70720745
CGTATCA39750.012.67189950
GCCGTAT40100.012.29957248
GCGTCGT42000.011.99434612
CGCTCGT48000.011.64404915
CGTATCG24500.011.5400923
CGCCGTA43600.011.3909147
CGAAGAT104400.011.13349151
CGCAGAC81200.010.9588671
GTATCGA31450.010.8536584
CGTTCGC20750.010.47492316
CGACCGT16950.010.3777321
CGCGTTC22900.010.2444314
CGATAAC26700.010.0759331
CGCGATT9850.09.80446151
CCGGGAT54900.09.7378111
TTACACG40850.09.71712232
TGGTCGC53950.09.52544343
TCGGGTC59250.09.4855122
Produced by FastQC (version 0.11.5)