FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32634443
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28100.019.76209643
TATGCCG36150.015.64769446
CGTATGC35300.015.53588144
TATCGCG16350.015.39992422
TTACGCG43550.014.96901154
GCGGTAT82500.014.4122021
TACGCGA47700.013.81129655
TATCTCG41600.013.431746539
CTCGTAT43300.013.22311142
GTTACGC54850.012.13668653
TCGACCG63750.011.6895631
AGTCACG49950.011.53397528
ACGCGAC58550.011.31081256
GATCGGA49050.011.3076181
ATCGGTA37850.011.29984918
ACACGTC52700.011.13333613
TAAACGC87500.011.11630752
CCAATCG39800.011.00626915
CGACCGT66550.010.938580532
GTTACGA27700.010.71515311
Produced by FastQC (version 0.11.5)