FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32634443
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG14550.021.10931422
GCGGTAT75650.014.4139151
GTATCGA31500.012.48811154
TTACGCG42750.012.01862954
TAAACGC81550.011.25187652
ATCGGTA37050.011.17321818
TCGACCG61000.011.08358331
CCCGTAT37150.010.7746451
TACGCGA47300.010.71556455
GTTACGA23350.010.63759611
GGTATCC103950.010.423553
CGACCGT61450.010.3848432
ATCGAAG69100.010.3367376
CCAATCG41850.010.22061115
CCGCAAT36350.010.1574141
GCGGAAT40050.010.0806181
CGTATCG39400.010.0719173
TGTTACG25600.09.83795310
GTATCCA119650.09.7766364
GCGCTTA34250.09.77389328
Produced by FastQC (version 0.11.5)