FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19303566
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG101450.031.96529843
TATGCCG104150.030.8705946
CGTATGC105850.030.6688344
CTCGTAT108550.029.04820642
ACACGTC117500.028.10094313
AGATCGG81450.027.6828691
GATCGGA122900.027.5898021
ATGCCGT120300.027.32848747
ACGTCTG122250.027.12134615
AATCTCG117050.027.05652439
TCTCGTA117500.026.39518741
ATCGGAA131150.025.849842
CACGTCT128550.025.79277814
ATCTCGT122500.025.57116140
GCCGTCT128600.025.34941349
TGCCGTC130100.025.08372548
CCGTCTT134550.024.27970350
TCGGAAG143350.023.8732993
GCACACG143100.023.62834411
CACACGT140400.023.56667512
Produced by FastQC (version 0.11.5)