FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19303566
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG64250.033.20297645
CGTATCA63550.032.48148750
CGCCGTA65950.032.19008347
GCCGTAT70150.030.4568748
TCGCCGT69400.029.74588246
GCGTCGT72800.029.50932112
TGGTCGC71150.028.96724543
CCGTATC75550.027.5959649
AGATCGG81150.027.2678571
GGTCGCC79150.026.95383644
AGCGTCG81000.026.81690211
CATACGA15400.025.07391715
CGTCGTG87600.024.52735313
CGGTGGT87250.024.10835540
TCTCGGT89950.024.0633437
AGAGCGT92800.023.4064469
GATCGGA95400.023.3715382
TCGGTGG97000.022.43285439
GTAGATC97700.022.3615332
GTGGTCG93250.022.28688442
Produced by FastQC (version 0.11.5)