FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23536288
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC277870.11806024807310311No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACT246670.10480412204337405No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA244780.10400110671657314No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG235450.10003701518268301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA66300.028.9914611
CGTCGAT69800.027.53773512
GCGTAGC93250.026.88742410
AGTGTCG91200.026.78260451
GTGCGTT92150.026.4326641
GGCTCGT76050.026.2560754
AGTGCGT95000.025.81872740
TCGTATG74900.025.79821843
GCCGCAA97750.024.8523634
AGGCGTA101000.024.7573878
TGTCGAG19250.024.4061015
GTGTCGA101300.024.38093652
CGTAGCC103650.024.1898611
CGTGCGT81150.024.1894578
TGTCGAT103250.023.9863753
CTCGGCT92450.023.8223881
TGCGTTC104200.023.66973942
CTCGTAT82300.023.395942
TAGCCCC108700.023.25382613
CGTATGC85750.022.6529744
Produced by FastQC (version 0.11.5)