FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29628125
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4052.3283064E-1013.6291731
GCGGTAT57300.012.8843861
ATCGGTA27900.012.6123118
TTACGCG33350.012.10533254
TAGCGAC32650.011.20214926
CCAATCG30050.011.13787215
TATCGCG9400.011.00945322
TACGCGA37550.010.84323355
CCGGGAT66600.010.5154141
CGTATCA24400.010.32282350
CGCGAAT10050.010.2981631
CCGGAAT75000.09.9816671
TAACGCC48500.09.88913529
GCGCGTA8400.09.8590669
TAAACGC62050.09.67604452
GTTACGC43650.09.64417853
TCGACCG39250.09.58217231
ACGCGAC43250.09.57374556
GCGCGAT22150.09.5008111
GGTATCC80350.09.4012083
Produced by FastQC (version 0.11.5)