FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36800255
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG125150.032.16759545
TATGCCG128500.030.84572248
CGTATGC131700.030.41056646
ACACGTC155950.028.53545213
AGATCGG102300.028.3353161
CTCGTAT143400.027.5685644
GATCGGA129350.026.1180441
ACGTCTG174650.025.53944815
CACGTCT175450.025.3836714
TCTCGTA166750.023.41850743
ATGCCGT168750.023.20225149
ATCTCGT170300.023.15328242
CACACGT199300.022.4844512
CGTCTGA219900.020.48791316
GCACACG226500.020.4392411
GCCGTCT194450.020.22466951
GTATGCC202800.020.03804647
ATCGGAA171700.019.6731642
AATCTCG180700.019.38790541
TCGGAAG181750.019.0598183
Produced by FastQC (version 0.11.5)