FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26142053
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG30450.010.869760514
CGAAGAT77600.010.7273621
CCGGGAT53150.010.7230491
TAAGCGA16550.010.24783546
CGCGTAT3854.509309E-59.8689191
CGGGATT25550.09.8689181
CTCGGAT66400.09.7277221
CGCGGAT22750.09.7170891
CGCGAAA12250.09.5714543
CTCAGAT156400.09.4966261
TATCGCG6554.3874024E-99.47522820
CTCGTTT39400.09.3804831
CTCGGGT62400.09.1888491
CGCCAAT46050.09.0009681
CGACAAT32050.08.945151
CTCGAAT46850.08.9209981
CGACTAT10200.08.8046231
CTTGGAT152100.08.7204651
CGCGGTT20450.08.614191
TAAGTCG18100.08.57220220
Produced by FastQC (version 0.11.5)