FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26142053
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT55500.012.2347011
CGAAGAT75300.011.9929621
TAAGCGA19450.011.1769346
TCGCTCG32850.010.70927614
CTCGAAT42000.09.7660251
CCGGCAT53550.09.5262581
CTCAGAT160800.09.4745021
TAAGTCG19800.09.40821220
CTCGGGT66650.09.360491
CGCAAAT24800.09.3120071
CTCAAAT156400.09.1019291
TCGGGTC52000.09.0823982
CGCGGTT19150.08.9995611
CCGCAAT31650.08.8212771
CGCAGAC76350.08.8033041
ACTCCGC32800.08.7234535
CGCGGAT22550.08.7126131
CAATCGG23800.08.6907414
CTCGGAT64300.08.6840871
AATCGGG27600.08.4934475
Produced by FastQC (version 0.11.5)