FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26895977
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA15000.017.03228815
CGGCATA19650.015.31743812
GCATACG20200.014.0338714
CGCGAAA12000.012.249907543
CGTTCGC18750.010.45249716
TCGGGTC48600.09.5783132
CGCGTTC20900.09.54492114
CTCGGGT64900.09.2756531
GTCCGAT14850.08.9560791
CGACCGT13750.08.9089411
TCGCGCG7503.6652636E-98.8665016
CTCGGAT70350.08.8555731
CGTATCG22050.08.7303043
CTCGTAT37850.08.692061
CCGGGAT47900.08.62198451
GTATCGA28600.08.5666394
CCGGCAT51200.08.5447781
CGCGGTC27450.08.5426611
TTCGCAC22700.08.47949818
CGAAGAT89100.08.4454251
Produced by FastQC (version 0.11.5)