FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27117437
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG85050.035.8550643
CGTATGC89300.033.99310744
TATGCCG92250.032.90606746
CTCGTAT92550.032.1667342
ACACGTC102850.030.11783213
GATCGGA102300.030.056151
CACGTCT119000.026.03041314
TCTCGTA114150.026.01997841
ATGCCGT124450.024.1425447
ACGTCTG129300.024.03687315
ATCTCGT125500.023.6943140
CACACGT131400.023.60021812
TCGGAAG133550.023.198263
GTAATCT134250.022.43264437
ATCGGAA139200.022.1578882
TAATCTC142650.021.16005538
GCCGTCT141950.021.04467449
AGATCGG74900.020.9653321
AATCTCG147150.020.48951739
TGCCGTC149000.020.25729648
Produced by FastQC (version 0.11.5)