FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24983949
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG158500.035.28191443
GATCGGA174150.032.257551
ACACGTC183200.032.20142413
CTCGTAT171050.032.12854442
AATCTCG171700.032.02700839
ACGTCTG190700.030.97116315
ATCGGAA183500.030.431442
TCTCGTA179350.030.27622241
TATGCCG184600.030.27482646
CGTATGC187800.029.79570244
ATCTCGT183150.029.74223340
CACGTCT200200.029.60489714
GCCGTCT198100.028.38582249
TCGGAAG200750.027.916613
CACACGT212000.027.90825712
GCACACG219600.027.09944211
CCGTCTT208950.026.96138250
CGTCTGA221200.026.7163216
ACCAATC210500.026.43518436
ATGCCGT212450.026.28987347
Produced by FastQC (version 0.11.5)