FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45984118
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA53850.013.19405750
TCGCTCG65600.013.12514
GCCGTAT59400.011.96127948
CGAAGAT161850.011.9153531
CGTATCG37000.011.4459473
CCGGGAT79800.011.359651
TTACACG54400.011.06617532
CGCAGAC120100.010.8118241
GTATCGA51800.010.5405414
CGCTCGT81750.010.5321115
TCGGGTC79250.010.3343852
GTCGCCG68000.010.19117745
CGCGTTC36300.010.12396714
CTCGGAT110900.09.9098281
CGCCGTA73300.09.74079147
CTCGGGT102900.09.6938771
GCGTCGT71200.09.68398912
TCGCGCG11650.09.6137338
CGTTCGC36450.09.60219516
CCGCGTT41600.09.33894313
Produced by FastQC (version 0.11.5)