FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26739754
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37050.013.9924346
TATGCCG38700.013.5721448
TTACACG27600.013.47117732
CGCGTAT4204.9294613E-1012.9998011
CTCGGGT54400.012.9221371
CGTATGC41250.012.5677747
GACGCTA25000.011.8689014
TATCTCG48500.011.32179741
ACACGTC50950.011.24658613
CCCGGTT32000.011.1970941
TCGGGTC45950.011.0606112
CGGGGTT62800.010.9763451
CTCGGAT61500.010.9309311
TAAGTCG23450.010.90811420
CGCGTAA3803.7982354E-610.7761511
CTCGGTT38900.010.7022781
GATCGGA47300.010.6728142
CTCGTTT44300.010.3220781
CGAAGAT93500.010.29208851
CTCGTAT53050.010.22220645
Produced by FastQC (version 0.11.5)