FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34790998
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38200.027.07431443
CGTATGC43550.024.30924644
TATGCCG49450.021.15897846
CTCGTAT46800.020.70704842
ACACGTC62500.018.20771213
GATCGGA61000.017.8847681
CGTATCG32750.013.8600233
CACGTCT84200.013.7565714
TCTCGTA73900.013.3340441
TTACGCG23050.013.222362554
ATGCCGT82800.013.04556447
GTATCGA34650.012.90457254
CCCGTAT37550.012.4516351
CGCGTAA34650.012.4181741
GCGGTAT55050.011.9399271
CACACGT97600.011.90256112
TACGCGA26100.011.80755
ACGTCTG100050.011.53926115
GTCGCTA25050.011.08273227
TCGGAAG100350.010.9707533
Produced by FastQC (version 0.11.5)